PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L
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PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L
Manufactured in an ISO 9001 and 13485 certified facility in the USA, ensuring quality and reliability for your molecular biology needs.
PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L
Numéro d'article: 127610908

PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

Numéro d'article: 127610908

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Ce qui se démarque

Versatile Buffer Solution
PBST 10X is ideal for a variety of lab applications, ensuring optimal pH levels to maintain the integrity of biological samples.
Detergent Inclusion
Contains 0.5% Tween-20, enhancing solubility and reducing non-specific binding in immunoassays, improving the accuracy of experimental results.
High Concentration
10X formulation allows for easy dilution, providing flexibility for different experimental needs while ensuring cost-effectiveness with a single concentrated solution.

Détails du produit

Shop PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L online at a best price in Vanuatu. B09V5NT2DQ
Container TypeBottle
Item FormLiquid
Item Weight16 ounces (453.6 grams)
Brand NameeCon Lab Supply
ManufacturerCiro Mfg
Included ComponentsBottle
Part Number23.105.11
BrandeCon Lab Supply
Package InformationBottle

À qui est-ce destiné ?

Suitable For
  • Laboratory Researchers

    Ideal for biologists conducting experiments requiring a buffered saline solution with surfactant properties for effective washing.

  • Immunoassay Users

    Excellent for those performing ELISA or Western blotting, where non-specific binding reduction is crucial.

  • Cell Culture Applications

    Useful for cell biologists needing a sterile environment and a reliable medium for maintaining cell integrity.

Not Suitable For
  • Environmental Testing

    Unsuitable for field applications where natural conditions and variations in salinity and pH are essential.

DESCRIPTION DU PRODUIT

PBST [10X]; Phosphate Buffered Saline with Tween-20 Solution; 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween-20, pH 7.4-1 L

Vous avez une question ? Chattez avec nous

Questions et réponses des clients

  • question: What is PBST and what is it used for?

    répondre: PBST, or Phosphate Buffered Saline with Tween-20, is a solution commonly used in biochemistry and molecular biology. It maintains a stable pH while providing an essential ionic environment for cells. This buffer also contains Tween-20, a non-ionic detergent, that is effective for blocking non-specific binding during various assays. Researchers often use PBST for washing in ELISA procedures, immunostaining, and other experiments requiring a stable buffered environment.
  • question: What is the impact of Tween-20 in PBST solutions?

    répondre: Tween-20 enhances the functionality of PBST by reducing non-specific binding sites, which is crucial during laboratory experiments where precision is vital. This detergent helps to maintain the structural integrity of proteins and other biological molecules while ensuring that only specific interactions occur during assays. For example, in ELISA tests, Tween-20 improves signal detection by decreasing background noise.
  • question: How should PBST be prepared and stored?

    répondre: PBST should ideally be prepared by diluting the 10X concentrated stock with distilled water to achieve the desired concentration. It should be stored at 4°C for short-term use or frozen for long-term storage. It is important to ensure the buffer is mixed thoroughly and, when using, to bring it to room temperature before use to avoid temperature-induced variations that can affect experimental results.
  • question: Can PBST be used in Western blotting?

    répondre: Yes, PBST is commonly used in Western blotting as a wash buffer and for diluting antibodies. Its composition helps to minimize background noise and enhances the clarity of the bands in the final visual results. For improved specificity in Western blots, PBST is often utilized after protein transfer processes to wash away unbound antibodies, ensuring that only those that specifically bind to the target protein remain.
  • question: What are the advantages of using a phosphate buffer in PBST?

    répondre: Using a phosphate buffer in PBST provides several advantages, primarily in maintaining a stable pH during various biological reactions. Phosphate buffers are compatible with many biological systems and minimize ionic strength fluctuations. This stability is critical for experiments involving proteins or nucleic acids that are sensitive to changes in pH, ensuring reliable and reproducible results in applications such as cell culture and various assay techniques.
  • question: Is PBST suitable for cell culture applications?

    répondre: PBST can be suitable for certain cell culture applications, especially those involving the washing or treatment of cells prior to further analysis. However, it’s essential to consider that while PBST maintains pH and reduces non-specific binding, it also contains Tween-20, which could affect cell viability. In cases where cell health is paramount, researchers often switch to a more benign buffer without detergents for handling live cell assays.
  • question: Can PBST be used in immunofluorescence staining?

    répondre: Absolutely, PBST is frequently utilized in immunofluorescence staining protocols. The buffer allows for effective permeabilization and reduces background fluorescence caused by non-specific antibody binding. Successfully utilizing PBST in the washing stages of immunofluorescence ensures that only the specific binding of antibodies to the target antigens is visualized, allowing clearer and more precise imaging results.
  • question: What precautions should be taken when using PBST?

    répondre: When using PBST, it’s important to check the expiration date as the buffer solutions can degrade over time. Additionally, to prevent contamination, always utilize sterile techniques when preparing and handling the buffer. If working with sensitive proteins or antibodies, ensure that the buffer components do not adversely affect stability or activity, as some proteins can be sensitive even to low concentrations of detergents.
  • question: Where can I buy PBST [10X]?

    répondre: You can purchase PBST [10X] from Ubuy in Vanuatu. They offer a wide range of laboratory reagents and supplies suitable for various research needs. Ubuy ensures that you receive high-quality products that meet industry standards, making it easier for you to obtain the essential materials for your experiments and research projects.
  • question: What is the shelf life of PBST?

    répondre: The shelf life of PBST can vary depending on the storage conditions and the presence of components like Tween-20. Generally, when stored correctly at 4°C, it can remain stable for several months. However, it's good practice to check for any visible signs of contamination or pH changes before use. Always maintain the buffer in clean, sterile containers to prolong its usability for your laboratory experiments.

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Caractéristiques et avantages

  • Comprehensive range of biological buffers for research and diagnostics.
  • Manufactured in a certified facility adhering to strict quality controls.
  • Offers 10X Concentrated solution of Phosphate Buffered Saline with Tween 20.
  • Ideal for wash solutions in Western blot and ELISA assays.
  • Formulated to effectively remove excess material without disrupting binding.
  • Ensures optimal performance in protein research and molecular biology studies.

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